Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes? Testing biochemistry.

Abstract:

This paper examines whether the in vivo behavior of yeast glycolysis can be understood in terms of the in vitro kinetic properties of the constituent enzymes. In nongrowing, anaerobic, compressed Saccharomyces cerevisiae the values of the kinetic parameters of most glycolytic enzymes were determined. For the other enzymes appropriate literature values were collected. By inserting these values into a kinetic model for glycolysis, fluxes and metabolites were calculated. Under the same conditions fluxes and metabolite levels were measured. In our first model, branch reactions were ignored. This model failed to reach the stable steady state that was observed in the experimental flux measurements. Introduction of branches towards trehalose, glycogen, glycerol and succinate did allow such a steady state. The predictions of this branched model were compared with the empirical behavior. Half of the enzymes matched their predicted flux in vivo within a factor of 2. For the other enzymes it was calculated what deviation between in vivo and in vitro kinetic characteristics could explain the discrepancy between in vitro rate and in vivo flux.

SEEK ID: https://sandbox9.fairdomhub.org/publications/7

PubMed ID: 10951190

Projects: SEEK Hands-On Session

Publication type: Not specified

Journal: Eur J Biochem

Citation: Eur J Biochem. 2000 Sep;267(17):5313-29.

Date Published: No date defined

Registered Mode: Not specified

Authors: B. Teusink, J. Passarge, C. A. Reijenga, E. Esgalhado, C. C. van der Weijden, M. Schepper, M. C. Walsh, B. M. Bakker, K. van Dam, H. V. Westerhoff, J. L. Snoep

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Created: 11th Apr 2018 at 12:30

Last updated: 9th Feb 2023 at 15:18

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